Multiple gene targeting siRNAs for down regulation of Immediate Early-2 (Ie2) and DNA polymerase genes mediated inhibition of novel rat Cytomegalovirus (strain All-03)

Table 5 Percentage knockdown of IE2 and DNA polymerase gene expression

Treatment	ΔCt expression	ΔΔCt expression	% knock down
A
dpb + dpc	0.094 ± 0.014	0.660 ± 0.104	33.90
dpb + ie2b	0.045 ± 0.001	0.318 ± 0.008	68.12
dpc + ie2b	0.051 ± 0.006	0.363 ± 0.046	63.67
dpb + dpc + ie2b	0.073 ± 0.008	0.518 ± 0.058	48.16
GCV	0.038 ± 0.001	0.270 ± 0.006	72.95
nc sirna	0.107 ± 0.014	1 ± 0.100	0
Virus control	0.142 ± 0.074	1 ± 0.518	0
B
dpb + dpc	0.029 ± 0.005	0.208 ± 0.041	79.11
dpb + ie2b	0.069 ± 0.013	0.485 ± 0.097	51.46
dpc + ie2b	0.044 ± 0.011	0.315 ± 0.078	68.41
dpb + dpc + ie2b	0.073 ± 0.014	0.518 ± 0.103	48.14
GCV	0.038 ± 0.001	0.270 ± 0.006	72.95
nc sirna	0.107 ± 0.014	1 ± 0.100	0
Virus control	0.142 ± 0.074	1 ± 0.518	0

All the experiments were conducted twice independently in triplicates were presented as results ± SEM. The results were calculated using delta delta Ct method and normalized to housekeeping gene, GAPDH mRNA. (A) IE2 and (B) DNA polymerase

ISSN: 1743-422X