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Table 4 Total amount of DNA copies in stock template

From: Multiple gene targeting siRNAs for down regulation of Immediate Early-2 (Ie2) and DNA polymerase genes mediated inhibition of novel rat Cytomegalovirus (strain All-03)

Sample

Viral DNA copies in ddPCR mixture

Viral DNA copies per µl (100 fold diluted)

Total viral DNA copies/ µl from stock template

Percentage of viral DNA copies reduction (%)

dpb + dpc

3.2

64

6400

68

dpb + ie2b

4

80

8000

60

dpc + ie2b

8.8

176

17,600

13

dpb + dpc + ie2b

5.9

118

11,800

42

gcv

6.1

122

12,200

40

nc siRNA

9

180

18,000

10

Untreated

10.1

202

20,200

0

Uninfected

0

0

0

0