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Table 3 Quantification Cycle (Cq) values of RT-qPCR compared to detection times of RT-LAMP detecting SARS-CoV-2 in pharyngeal swab samples

From: Rapid point-of-care detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP)

Pharyngeal swab

Cq value RT-qPCR

Orf1a

Detection time

RT-LAMP

ORF8

Detection time

RT-LAMP

N

Detection time

RT-LAMP

RNase P (IC)

Positive 1

18.40

9.55

12.67

22.78

Positive 2

24.13

11.48

15.24

23.22

Positive 3

28.97

11.93

17.84

19.34

Positive 4

29.26

14.19

17.39

20.30

Positive 5

30.47

15.05

19.34

20.98

Negative 1

   

20.97

Negative 2

   

21.06

Negative 3

   

21.69

Negative 4

   

23.96

Negative 5

   

15.18

  1. Ten pharyngeal swab samples were routinely tested for COVID-19 using ampliCube Coronavirus SARS-CoV-2 kit acquired from Mikrogen Diagnostik, where Orf1a serves as specific target for SARS-CoV-2. Positive samples 1–5 and Negative samples 1–5 were pre-heated at 90 °C for 5 min and directly tested for SARS-CoV-2 in RT-LAMP. The targets ORF8 and N detect SARS-CoV-2, while RNase P is used as internal control (IC) for proof of human material in the swab
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Virology Journal

ISSN: 1743-422X