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Table 3 Quantification Cycle (Cq) values of RT-qPCR compared to detection times of RT-LAMP detecting SARS-CoV-2 in pharyngeal swab samples

From: Rapid point-of-care detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP)

Pharyngeal swab Cq value RT-qPCR
Orf1a
Detection time
RT-LAMP
ORF8
Detection time
RT-LAMP
N
Detection time
RT-LAMP
RNase P (IC)
Positive 1 18.40 9.55 12.67 22.78
Positive 2 24.13 11.48 15.24 23.22
Positive 3 28.97 11.93 17.84 19.34
Positive 4 29.26 14.19 17.39 20.30
Positive 5 30.47 15.05 19.34 20.98
Negative 1     20.97
Negative 2     21.06
Negative 3     21.69
Negative 4     23.96
Negative 5     15.18
  1. Ten pharyngeal swab samples were routinely tested for COVID-19 using ampliCube Coronavirus SARS-CoV-2 kit acquired from Mikrogen Diagnostik, where Orf1a serves as specific target for SARS-CoV-2. Positive samples 1–5 and Negative samples 1–5 were pre-heated at 90 °C for 5 min and directly tested for SARS-CoV-2 in RT-LAMP. The targets ORF8 and N detect SARS-CoV-2, while RNase P is used as internal control (IC) for proof of human material in the swab