Detection and identification of enteroviruses circulating in children with acute gastroenteritis in Pará State, Northern Brazil (2010–2011)

Table 3 Sequences of the Primers used in Real-Time PCR, cDNA synthesis, PCR amplification and sequencing for EV detection

Primer/ Probe	Sequence^a	Gene	Location^b
EVReal T(A) ^c	GCGATTGTCACCATWAGCAGYCA	5′-UTR	599–577
EVRealT(S)^c	GGCCCCTGAATGCGGCTAATCC	5′-UTR	449–470
PanEVProbe (S)^c	FAM-CCGACTACTTTGGGWGTCCGTGT-MGBNFQ	5′-UTR	537–559
AN32^d	GTYTGCCA	VP1	3009–3002
AN33 ^d	GAYTGCCA	VP1	3009–3002
AN34 ^d	CCRTCRTA	VP1	3111–3104
AN35 ^d	RCTYTGCCA	VP1	3009–3002
222 ^d	CICCIGGIGGIAYRWACAT	VP1	1977–1996
224 ^d	GCIATGYTIGGIACICAYRT	VP3	2969–2951
292 ^d	MIGCIGYIGARACNGG	VP1	2612–2627
AN88 ^d	TACTGGACCACCTGGNGGNAYRWACAT	VP1	2977–2951
AN89 ^d	CCAGCACTGACAGCAGYNGARAYNGG	VP1	2602–2627

^aDegenerate primers: Y = C or T; R = A or G; W = A or T; M = A or C; N = A, C, G or T; I = Inosine;
^bThe locations of all primers are relative to the genome of poliovirus type 1, Mahoney strain;
^c[17];
^d[14, 16]

ISSN: 1743-422X