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Fig. 4 | Virology Journal

Fig. 4

From: Protective CD8+ T-cell response against Hantaan virus infection induced by immunization with designed linear multi-epitope peptides in HLA-A2.1/Kb transgenic mice

Fig. 4

The proliferation of HTNV-specific CD8+T-cells stimulated by peptide in HLA-A2.1/Kb Tg mice with different immunization. Representative flow cytometric histograms of the expansion percentage of HTNV-specific CD8+ T-cells stimulated by peptides in each immunized Tg mice group, including immunization with HTNV linear multi-epitope peptide PADRE-VV9, HLA-A*02-restricted single HTNV-GP CTL epitope VV9, HLA-B*35-restricted HTNV-NP CTL epitope VY9 (unrelated peptide control), the HTNV-inactivated vaccine (positive control) and PBS injection (negative control), respectively. Each group containing three conditions in histograms clearly demonstrated that the extent of CFSE curve shifted to the left represented the percentage of CD8+ T-cell proliferation. The middle row shows the epitope VV9 stimulation. The upper and the lower rows showed the PHA stimulation as positive control and no stimuli as negative control, respectively. The analysis of the cells was carried out using gated CD3+CD8+ T-cells. CFSE, 5, 6-carboxyfluorescein succinimidyl ester. PHA, phytohemagglutinin. PBS, phosphate buffer saline. VV9, VMASLVWPV. VY9, VPILLKALY. PADRE, AKXVAAWTLKAAA, X = cyclohexylalanine

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