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Fig. 1 | Virology Journal

Fig. 1

From: RNA silencing-related genes contribute to tolerance of infection with potato virus X and Y in a susceptible tomato plant

Fig. 1

a Schematic diagram of the artificial chimeric genes (AGO2 and 3). Based on the alignment of the sequences of three tomato AGO genes (AGO2a, b and AGO3) registered in the database, three regions in AGO, i.e., one from AGO2a (270 bp), one from AGO2b (283 bp), and one from AGO3 (295 bp) were selected. The chimeric gene constructs were subsequently placed in head-to-head orientation across an intron sequence (‘int’ in the figure) to create an IR sequence. b Relative expression levels of the DCLs, AGOs, and RDR6 mRNAs in transgenic S. lycopersicum plants expressing dsRNAs, compared with empty vector-transformed plants. The relative levels of these mRNAs were investigated through real-time reverse transcription-polymerase chain reaction (RT-PCR) using 18S ribosome RNA as a control. Error bars represent SE. Student’s t-test was applied to analyze the data, comparing dsRNA-expressing and empty vector-transformed plants. The assay was repeated twice, and each analysis consisted of three biological replicates from three plants per treatment. Asterisks indicate a statistically significant difference in the accumulation of gene mRNA between plants with or without the expression of dsRNA (p < 0.05)

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