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Fig. 1 | Virology Journal

Fig. 1

From: Role of HVR1 sequence similarity in the cross-genotypic neutralization of HCV

Fig. 1

Monovalent vaccinations elicits high-titre, immunogen specific Ab. a HVR1 variability in a GenBank reference set was visualized by Shannon Entropy. Higher entropy corresponds to positions of greater variation. b Candidate immunogens (I.1, 1.2) differ at 14/21 amino acids. Conserved positions are indicated by asterisk. c Vaccination protocol for I.1 and 1.2 immunogens. Groups of 4, female Balb/C mice were vaccinated with either immunogen I.1, I.2, or adjuvant alone. Mice were bled on day 48, with group sera pooled for subsequent assays. d An ELISA plate was coated with immunogen I.1 (left panel), I.2 (middle panel), or both I.1/I.2 (right panel). Heat-inactivated mouse sera from each vaccine group was added at the indicated concentrations. e Separate rows of an ELISA plate were coated with either I.1-peptide (unconjugated), KLH (+), or BSA (−). Heat-inactivated mouse sera was added at 1:1000 dilution. For both ELISA, the binding of antibody was detected with anti-mouse secondary antibody. Averages of data from triplicates are shown. Statistical analysis was done by one-tailed, unpaired t test. *P < 0.001

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