Fig. 3
From: HLA-associated protection of lymphocytes during influenza virus infection
Influenza Virus (IAV)-infected HLA-A2-homozygous Lymphocytes Serve as Infectious Foci for Uninfected Monocytes-macrophages. This representative image shows results using cells from HLA-A2-homozygous donor number 2. Autoradiograms show representative protein synthesis results using PBMC from an HLA-A2-homozygous donor. After sham-exposure (lanes 1 and 3) or exposure to IAV at an MOI = 10 (lanes 2 and 4), purified macrophages (lanes 1 and 2) and lymphocytes (lanes 3 and 4) were obtained and pulse-labeled. Additional aliquots of virus-exposed lymphocytes, after separation from macrophages by elutriation, were treated with neuraminidase, washed, and layered over additional aliquots of autologous control macrophages. After 1 h, the latter macrophages were extensively washed free of lymphocytes, pulse-labeled, and analyzed (lane 5)