Fig. 2
From: HLA-associated protection of lymphocytes during influenza virus infection
Influenza Virus (IAV) Neuraminidase (NA) RNA is Synthesized by HLA-A2- heterozygous (HTZ) but not HLA-A2-homozygous (HMZ) Lymphocytes. This representative image shows results using cells from HLA-A2-homozygous donor number 3. Autoradiograms of Northern blots show cell lysates from an HLA-A2-HMZ donor (lanes 1–10) and an HLA-A1,2 donor (lanes 11–20) obtained after sham-exposure (odd-numbered lanes) or exposure to IAV (even-numbered lanes). After exposure as unseparated PBMC, purified lymphocytes were obtained and lysates were collected after 2 h (lanes 1, 2 and 11, 12), 4 h (lanes 3, 4 and 13, 14), 8 h (lanes 5, 6 and 15, 16), and 24 h (lanes 7, 8 and 17, 18). Lysates of purified monocytes/macrophages (lanes 9, 10 and 19, 20) were collected 4 h after exposure. Lysates were probed for positive strand NA as described in the Methods. The original autoradiogram of the HLA-A1,2 donor’s lysates also showed a faint NA signal in lane 16 as well as 12 and 14