Fig. 5
From: A novel two-step, direct-to-PCR method for virus detection off swabs using human coronavirus 229E
Gel visualization of the amplicons resulting from RT-qPCR done for method sensitivity testing at the lower limit of detection shown in Fig. 4. Visualization of a 308 bp amplicon is confirmatory of the HCoV-229E N gene. Note, of all 56 samples shown, only two swab samples (red box) and the one negative control (blue box) seen on the final gel of the series did not validate with amplicon visualization. All other unlabeled lanes are viral lysate produced N gene amplicon