Fig. 4From: A novel two-step, direct-to-PCR method for virus detection off swabs using human coronavirus 229EHCoV-229E N transcript detection using RT-qPCR following shaker-mill homogenization off swabs spiked in vitro with 1.2 × 103 viral copies/mL. Orange lines represent viral stock 1 spiked swabs at 1.2 × 102 viral copies/mL. Grey lines represent viral stock 2 spiked swabs at 1.2 × 102 viral copies/mL. The green line represents a negative control, virus-free swabBack to article page