Fig. 3
From: A novel two-step, direct-to-PCR method for virus detection off swabs using human coronavirus 229E
Gel visualization of amplicons from RT-qPCR shown in Fig. 2. Starting at the furthest left lane on the first gel, lane 1 is 100 bp DNA ladder, lanes 2 and 3 are amplicon visualization of 1.2 × 107 viral copies/mL swab spike, lanes 4,5, and 6 are amplicon visualization of 1.2 × 106 viral copies/mL swab spike, lanes 7,8, and 9 are amplicon visualization of 1.2 × 105 viral copies/mL swab spike, lanes 10,11, and 12 are amplicon visualization of 1.2 × 104 viral copies/mL swab spike, lanes 13,14,15 are empty. Starting with the furthest left lane on the second gel, lane 1 is 100 bp DNA ladder, lanes 2, 3, and 4 are amplicon visualization of 1.2 × 103 viral copies/mL swab spike, lanes 5,6, and 7 are amplicon visualization of 1.2 × 102 viral copies/mL swab spike, lanes 8, 9, and 10 are amplicon visualization of 1.2 × 101 viral copies/mL swab spike, lanes 11,12, 13, 14, and 15 are empty. Visualization of a 308 bp amplicon is confirmatory of the HCoV-229E N gene