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Fig. 5 | Virology Journal

Fig. 5

From: MMPphg from the thermophilic Meiothermus bacteriophage MMP17 as a potential antimicrobial agent against both Gram-negative and Gram-positive bacteria

Fig. 5

Dependence of MMPphg bacteriolytic and bactericidal activity on its concentration. a Turbidity reduction assays. Different concentrations of MMPphg were incubated with Salmonella ser. Paratyphi B at 37 °C, and the changes in OD600 were measured over time. b Bactericidal activity of MMPphg against Salmonella ser. Paratyphi B. After incubation at 37 °C over 60 min period (samples were taken at an interval of 10 min), aliquots were serially diluted and spread onto LB agar plates. Log kills were presented as ΔLog10 (CFU/mL) compared with bacterial counts before treatment. Kanamycin (50 μg/mL, approximately 86 μM) and PBS were used as the controls. Data were presented as mean ± SD, and all experiments were repeated in triplicate

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