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Fig. 6 | Virology Journal

Fig. 6

From: AIV polyantigen epitope expressed by recombinant baculovirus induces a systemic immune response in chicken and mouse models

Fig. 6

THB expressed in the splenocytes of the BmNPV-CMV/THB-P10/CTLT-injected mice and the level of anti-THB antibody detection. a THB expressed in mouse splenocytes. The spleens were dissected from the mice at 48 h post-vaccination with BmNPV-CMV/THB-P10/CTLT, and immunohistochemistry was performed with the mouse anti-H7H9 HA (primary antibody) and FITC-labeled goat anti-mouse IgG (secondary antibody). The cell nucleus was stained with DAPI. CK, normal mouse; Test, the mice vaccinated with BmNPV-CMV/THB-P10/CTLT. b The level of anti-THB antibody detected in the serum of the vaccinated mice with BmNPV-CMV-THB-CTLT. The mice were vaccinated with BmNPV-CMV-THB-CTLT at a dose of 1010 TCID50/kg (Group 1), 5 × 109 TCID50 /kg (Group 2), and 109 TCID50/kg (Group 3). The serum sampled at 1, 2, 3, and 4 weeks post-vaccination were used to determine the level of anti-THB antibodies by indirect ELISA

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Virology Journal

ISSN: 1743-422X