Fig. 5
A specific immune response against THB could be generated in the chickens vaccinated with BmNPV-CMV/THB-P10/CTLT. a The detected levels of anti-THB antibodies in the serum of the vaccinated chickens with BmNPV-CMV-THB-CTLT. The chickens were vaccinated with BmNPV-CMV-THB-CTLT at a dose of 1012 TCID50/kg (Group 1), 5 × 1011 TCID50 /kg (Group 2), and 1011 TCID50/kg (Group 3). The serum samples at 1, 2, 3, 4, and 5 weeks post-vaccination were used to determine the level of anti-THB antibodies via an indirect ELISA. b The splenic T lymphocyte proliferative responses in vaccinated chickens. The lymphocyte cell suspension was stimulated with ConA (5 μg/mL final concentration) and H7HA standard antigen (0.5 μg/mL final concentration) was seeded into a 96-well plate at 100 μL per well. An unstimulated cellular suspension was used as a control. The cells were incubated at 5% CO2 and 39.5 °C for 44 h. The cell number was determined using the MTT method. *P < 0.05