Skip to main content
Fig. 3 | Virology Journal

Fig. 3

From: AIV polyantigen epitope expressed by recombinant baculovirus induces a systemic immune response in chicken and mouse models

Fig. 3

THB expression in BmNPV-CMV/THB-P10/CTLT-infected HEK293T cells. a PCR detection of the BmNPV-CMV/THB-P10/CTLT in the HECK293T cells. Lane M: DNA marker; Lane CTLT: PCR product of CTLC; Lane CMV: PCR product of the CMV promoter; lane THB: PCR product of THB. b THB transcript detected by RT-PCR. Lane WT: BmNPV-infected HECK293T cells; lane THB: BmNPV-CMV/THB-P10/CTLT-infected HECK293T cells. c The expression of THB in the BmNPV-CMV/THB-P10/CTLT-infected HEK293T cells was detected by immunohistochemistry. 1, 1′ and 1″, BmNPV-CMV/THB-P10/CTLT-infected BmN cells; 2, 2’and 2″, normal BmN cells; 1 and 2, cell nucleus was stained by DAPI; 1′ and 2′, the THB stained by Cy3; 1″, the merger of 1 and 1′; 2″, the merger of 2 and 2′. Mouse anti-H7H9 HA was used as the primary antibody; Cy3-labbled goat anti-mouse IgG was used as a secondary antibody

Back to article page