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Fig. 2 | Virology Journal

Fig. 2

From: AIV polyantigen epitope expressed by recombinant baculovirus induces a systemic immune response in chicken and mouse models

Fig. 2

CTLT expressed in BmNPV-CMV/THB-P10/CTLT-infected BmN cells. a CTLT transcript detected by RT-PCR; Lane M: DNA marker; Lane 1: CTLT transcript. b CTLT expressed in the BmNPV-CMV/THB-P10/CTLT-infected BmN cells was detected by Western blot; lane 1: BmN cells infected with BmNPV-CMV/THB-P10/CTLT; lane 2: BmN cells infected with BmNPV; lane 3: normal BmN cells. Primary antibody; lane M: protein marker. Mouse anti-H7H9 HA was used as a primary antibody; HRP-conjugated goat anti-mouse IgG was used as a secondary antibody. c, CTLT expressed in BmNPV-CMV/THB-P10/CTLT-infected BmN cells was detected by immunohistochemistry. 1, 1′ and 1″, normal BmN cells; 2, 2’and 2″, BmNPV-CMV/THB-P10/CTLT infected BmN cells; 1 and 2, the cell nucleus was stained by DAPI; 1′ and 2′, CTLT stained by Cy3; 1″, the merger of 1 and 1′; 2″, the merger of 2 and 2′. Mouse anti-H7H9 HA was used as a primary antibody; Cy3-labeled goat anti-mouse IgG was used as a secondary antibody

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Virology Journal

ISSN: 1743-422X