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Fig. 1 | Virology Journal

Fig. 1

From: AIV polyantigen epitope expressed by recombinant baculovirus induces a systemic immune response in chicken and mouse models

Fig. 1

Construction of the Baculovirus transfer vector, pFastBac™DuaI-CMV/THB-P10/CTLT, and identification of the recombinant Baculovirus BmNPV-CMV/THB-P10/CTLT. A, Construction of the Baculovirus transfer vector, pFastBac™DuaI-CMV/THB-P10/CTLT, a coding sequence (CTLT) of T lymphocyte epitopes from H1HA, H9HA, and H7HA AIV subtypes was controlled by the baculovirus P10 promoter, and a coding sequence (THB) of B cell epitopes from the H1HA, H9HA, and H7HA AIV subtypes was driven by the CMV promoter. B-1, control BmN cells; B-2, the recombinant Baculovirus BmNPV-CMV/THB-P10/CTLT-infected BmN cells. C-1, the CMV promoter amplified from the recombinant Baculovirus BmNPV-CMV/THB-P10/CTLT; lane M: DNA marker; lane 1: BmNPV; and lanes 2–4: P1, P2, and P3 BmNPV-CMV/THB-P10/CTLT. C-2, the THB fragment amplified from the recombinant Baculovirus BmNPV-CMV/THB-P10/CTLT; lane M: DNA marker; lanes 1–3: P1, P2, and P3 BmNPV-CMV/THB-P10/CTLT; lane 4: pFastBacTMDuaI-CMV/THB-P10/CTLT; lane 5: BmNPV. C-3, the CTLT fragment amplified from the recombinant Baculovirus BmNPV-CMV/THB-P10/CTLT; lane M: DNA marker; lanes 1–3: P1, P2, and P3 BmNPV-CMV/THB-P10/CTLT; lane 4: pFastBacTMDuaI-CMV/THB-P10/CTLT; lane 5: BmNPV

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Virology Journal

ISSN: 1743-422X