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Table 1 Oligonucleotide primers used in this study

From: Identification of the largest non-essential regions of the C-terminal portion in 3A protein of foot-and-mouth disease virus for replication in cell culture

Primer Sequence(5′ → 3′) usage
HN-1F CAAGAAGTGATTGAGCGGGT  
3A76–153F TGGCAAACATAGTGATCATGGGACCCTACGCCGGGCCACT △76–153
3A76–153R AGTGGCCCGGCGTAGGGTCCCATGATCACTATGTTTGCCA △76–153
3A77–153F CAAACATAGTGATCATGCTAGGACCCTACGCCGGGCCACT △77–153
3A77–153R AGTGGCCCGGCGTAGGGTCCTAGCATGATCACTATGTTTG △77–153
3A80–153F TGATCATGCTACGCGAAGCGGGACCCTACGCCGGGCCACT △80–153
3A80–153R AGTGGCCCGGCGTAGGGTCCCGCTTCGCGTAGCATGATCA △80–153
3A76V-153F CAAACATAGTGATCATGGTAGGACCCTACGCCGGGCCACT △76 V-153
3A76V-153R AGTGGCCCGGCGTAGGGTCCTACCATGATCACTATGTTTG △76 V-153
3A76I-153F CAAACATAGTGATCATGATAGGACCCTACGCCGGGCCACT △76I-153
3A76I-153R AGTGGCCCGGCGTAGGGTCCTATCATGATCACTATGTTTG △76I-153
3A-GFP1F AAGCGCGCAAGAGGCGCCAGATGGTGAGCAAGGGCGAGGA ▽GFP
3A-GFP1R TCCTCGCCCTTGCTCACCATCTGGCGCCTCTTGCGCGCTT ▽GFP
3A-GFP2F GCATGGACGAGCTGTACAAGGGACCCTACGCCGGGCCACT ▽GFP
3A-GFP2R AGTGGCCCGGCGTAGGGTCCCTTGTACAGCTCGTCCATGC ▽GFP
HN-4R GTTCCCTTCTTCATTCTCGC  
  1. △indicates amino acids deletion. ▽indicates amino acids insertion
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Virology Journal

ISSN: 1743-422X