Identification of the largest non-essential regions of the C-terminal portion in 3A protein of foot-and-mouth disease virus for replication in cell culture

Table 1 Oligonucleotide primers used in this study

Primer	Sequence(5′ → 3′)	usage
HN-1F	CAAGAAGTGATTGAGCGGGT
3A_76–153F	TGGCAAACATAGTGATCATGGGACCCTACGCCGGGCCACT	△76–153
3A_76–153R	AGTGGCCCGGCGTAGGGTCCCATGATCACTATGTTTGCCA	△76–153
3A_77–153F	CAAACATAGTGATCATGCTAGGACCCTACGCCGGGCCACT	△77–153
3A_77–153R	AGTGGCCCGGCGTAGGGTCCTAGCATGATCACTATGTTTG	△77–153
3A_80–153F	TGATCATGCTACGCGAAGCGGGACCCTACGCCGGGCCACT	△80–153
3A_80–153R	AGTGGCCCGGCGTAGGGTCCCGCTTCGCGTAGCATGATCA	△80–153
3A_76V-153F	CAAACATAGTGATCATGGTAGGACCCTACGCCGGGCCACT	△76 V-153
3A_76V-153R	AGTGGCCCGGCGTAGGGTCCTACCATGATCACTATGTTTG	△76 V-153
3A_76I-153F	CAAACATAGTGATCATGATAGGACCCTACGCCGGGCCACT	△76I-153
3A_76I-153R	AGTGGCCCGGCGTAGGGTCCTATCATGATCACTATGTTTG	△76I-153
3A-GFP1F	AAGCGCGCAAGAGGCGCCAGATGGTGAGCAAGGGCGAGGA	▽GFP
3A-GFP1R	TCCTCGCCCTTGCTCACCATCTGGCGCCTCTTGCGCGCTT	▽GFP
3A-GFP2F	GCATGGACGAGCTGTACAAGGGACCCTACGCCGGGCCACT	▽GFP
3A-GFP2R	AGTGGCCCGGCGTAGGGTCCCTTGTACAGCTCGTCCATGC	▽GFP
HN-4R	GTTCCCTTCTTCATTCTCGC

ISSN: 1743-422X