Table 1 Primer and probe sequence information to detect influenza virus type (A/B) and subtype (H1/H3)
Type/Subtype | Primer/probe | Sequence (5′–3′) | Target (bp) |
|---|---|---|---|
A | Forward | AATCCTGTCACCTCTGACTAAGG | Matrix (98) |
Reverse | CATTYTGGACAAAKCGTCTACG | ||
Probe | FAM-TGCAGTCCTCGCTCAC-MGBNFQ | ||
B | Forward | GAATGCTGTCAATGAATATTGAGGG | Nucleoprotein (77) |
Reverse | CATTGAGTCATTCATCATCTTGAGTAGAT | ||
Probe | VIC-TCCTTTGACATCTGCAT-MGBNFQ | ||
H1 | Forward | AACAATTCAACAGACACTGTAGACACAGT | Hemagglutinin (144) |
Reverse | GGGCTACCCCTCTTAGTTTRCATAGTTT | ||
Probe | FAM- ATGTAACAGTAACACACTCTGT-MGBNFQ | ||
H3 | Forward | GGAATGGTTTGTCATTGGGAAT | Hemagglutinin (95) |
Reverse | AAGCTCAATAATGAGRTCAGATGCA | ||
Probe | VIC-CTTCCATTTGGAGTRATRCA-MGBNFQ | ||
IPC | Forward | AGATTTGGACCTGCGAGCG | GAPDH (65) |
Reverse | GAGCGGCTGTCTCCACAAGT | ||
Probe | Cy5-TTCTGACCTGAAGGCTCTGCGCG-BHQ |