Table 2 Comparison of test results of both LAMP and PCR & Sequencing in clinical sample detection: The PCR & Sequencing method was used as the standard, the sensitivity and specificity of the microfluidic LAMP method used to detect the clinical samples were analyzed
LAMP | PCR | Total | Sensitive (%) | Specificity (%) | Kappa | |
|---|---|---|---|---|---|---|
Positive | Negative | 95% CI | 95% CI | |||
HPV27 | ||||||
 Positive | 30 | 36 | 66 | 100 (88.43–100) | 34.55 (22.24–48.58) | 0.271 (P < 0.001) |
 Negative | 0 | 19 | 19 | |||
 Total | 30 | 55 | 85 | |||
HPV2 | ||||||
 Positive | 3 | 45 | 48 | 100 (29.24–100) | 45.12 (34.1–56.51) | 0.055 (P > 0.05) |
 Negative | 0 | 37 | 37 | |||
 Total | 3 | 82 | 85 | |||
HPV1 | ||||||
 Positive | 13 | 3 | 16 | 100 (75.29–100) | 95.83 (88.3–99.13) | 0.876 (P < 0.001) |
 Negative | 0 | 69 | 69 | |||
 Total | 13 | 72 | 85 | |||
HPV57 | ||||||
 Positive | 14 | 1 | 15 | 100 (76.84–100) | 98.59 (92.40–99.96) | 0.958 (P < 0.001) |
 Negative | 0 | 70 | 70 | |||
 Total | 14 | 71 | 85 | |||
HPV3 | ||||||
 Positive | 1 | 2 | 3 | 100 (2.5–100) | 97.62 (91.66–99.71) | 0.491 (P < 0.001) |
 Negative | 0 | 82 | 82 | |||
 Total | 1 | 84 | 85 | |||
HPV4 | ||||||
 Positive | 0 | 3 | 3 | – | 96.47 (90.03–99.27) | – |
 Negative | 0 | 82 | 82 | |||
 Total | 0 | 85 | 85 | |||
HPV7 | ||||||
 Positive | 0 | 1 | 1 | – | 98.82 (93.62–99.97) | – |
 Negative | 0 | 84 | 84 | |||
 Total | 0 | 85 | 85 | |||
HPV75 | ||||||
 Positive | 0 | 1 | 1 | – | 98.82 (93.62–99.97) | – |
 Negative | 0 | 84 | 84 | |||
 Total | 0 | 85 | 85 | |||