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Table 2 Comparison of test results of both LAMP and PCR & Sequencing in clinical sample detection: The PCR & Sequencing method was used as the standard, the sensitivity and specificity of the microfluidic LAMP method used to detect the clinical samples were analyzed

From: Genotyping of 30 kinds of cutaneous human papillomaviruses by a multiplex microfluidic loop-mediated isothermal amplification and visual detection method

LAMP PCR Total Sensitive (%) Specificity (%) Kappa
Positive Negative 95% CI 95% CI
HPV27
 Positive 30 36 66 100 (88.43–100) 34.55 (22.24–48.58) 0.271 (P < 0.001)
 Negative 0 19 19
 Total 30 55 85
HPV2
 Positive 3 45 48 100 (29.24–100) 45.12 (34.1–56.51) 0.055 (P > 0.05)
 Negative 0 37 37
 Total 3 82 85
HPV1
 Positive 13 3 16 100 (75.29–100) 95.83 (88.3–99.13) 0.876 (P < 0.001)
 Negative 0 69 69
 Total 13 72 85
HPV57
 Positive 14 1 15 100 (76.84–100) 98.59 (92.40–99.96) 0.958 (P < 0.001)
 Negative 0 70 70
 Total 14 71 85
HPV3
 Positive 1 2 3 100 (2.5–100) 97.62 (91.66–99.71) 0.491 (P < 0.001)
 Negative 0 82 82
 Total 1 84 85
HPV4
 Positive 0 3 3 96.47 (90.03–99.27)
 Negative 0 82 82
 Total 0 85 85
HPV7
 Positive 0 1 1 98.82 (93.62–99.97)
 Negative 0 84 84
 Total 0 85 85
HPV75
 Positive 0 1 1 98.82 (93.62–99.97)
 Negative 0 84 84
 Total 0 85 85
  1. LAMP represents the new HPV detection system based on LAMP and microfluidic chip. PCR represents DNA sequencing based on PCR method
  2. Abbreviations: HPV human papillomaviruses, LAMP Loop-mediated isothermal amplification, PCR Polymerase Chain Reaction, CI confidence interval