Fig. 3
30 Kinds of Cutaneous HPV sensitivity tests. In one chip, LAMP reactions were performed between 6 HPV type primers and a mixture of these 6 HPV type plasmids at a certain concentration. When the first 6 wells are positive (sky blue) and the two NC wells are negative (violet), it means that these 6 HPV primers can produce a positive reaction with this concentration of HPV type plasmid. Positive amplifications occurred at a plasmid concentration of 107 copies/μl for all primers tested, but not all primers showed positive reactions at 106 copies/μl. The lowest concentration of HPV DNA that the detection system can detect was set to 107 copies/μl. a Sensitivity detection of group 1 in the low risk type group. The HPV plasmid concentrations of 108, 107 and 106 copies/μl were selected for sensitivity detection of HPV primers in group 1of low risk type group. b Sensitivity detection of group 2 in the low risk type group. The HPV plasmid concentrations of 108, 107 and 106 copies/μl were selected for sensitivity detection of HPV primers in group 2 of low risk type group. c Sensitivity detection of group 3 in the low risk type group. The HPV plasmid concentrations of 108, 107 and 106 copies/μl were selected for sensitivity detection of HPV primers in group 3 of low risk type group. d Sensitivity detection of high risk type group. The HPV plasmid concentrations of 108, 107 and 106 copies/μl were selected for sensitivity detection of HPV primers in high risk type group. e Sensitivity detection of rare type group. The HPV plasmid concentrations of 108, 107 and 106 copies/μl were selected for sensitivity detection of HPV primers in rare type group