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Fig. 2 | Virology Journal

Fig. 2

From: Genotyping of 30 kinds of cutaneous human papillomaviruses by a multiplex microfluidic loop-mediated isothermal amplification and visual detection method

Fig. 2

Specificity tests among three low-risk type HPV groups. In one chip, LAMP reactions were performed between the 7 HPV type primers and the HPV type plasmid of the 7th well. When the first 6 wells and one NC well are negative (violet) and the 7th well is positive (sky blue), it means that the HPV type primers of the first 6 wells are specific to the HPV type plasmid of the 7th well. When a positive reaction occurs in the first 6 wells, it means that the HPV type primer in this well has a non-specific cross-reaction with the HPV type plasmid in the 7th well. a Specificity detection of group 1 against group 2 and 3. Using group 2 and 3 HPV plasmids to detect the specificity of group 1 HPV primers. b Specificity detection of group 2 against group 1 and 3. Using group 1 and 3 HPV plasmids to detect the specificity of group 2 HPV primers. c Specificity detection of group 3 against group 1 and 2. Using group 1 and 2 HPV plasmids to detect the specificity of group 3 HPV primers

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