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Fig. 1 | Virology Journal

Fig. 1

From: Genotyping of 30 kinds of cutaneous human papillomaviruses by a multiplex microfluidic loop-mediated isothermal amplification and visual detection method

Fig. 1

Specificity tests within groups. In one chip, LAMP reactions were performed between the six HPV type primers and the HPV type plasmid in the sixth well. When the first 5 wells and two NC wells are negative (violet) and the 6th well is positive (sky blue), it means that the HPV type primers of the first 5 wells have good specificity for the HPV type plasmid of the 6th well. a Specificity detection of group 1 in the low risk type group. The specificity of the six HPV type primers (HPV1, 2, 4, 7, 10 and 57) in the group was verified. b Specificity detection of group 2 in the low risk type group. The specificity of the six HPV type primers (HPV3, 27, 41, 49, 65 and 75) in the group was verified. c Specificity detection of group 3 in the low risk type group. The specificity of the six HPV type primers (HPV28, 29, 76, 77, 117 and 125) in the group was verified. d Specificity detection of high risk type group. The specificity of the six HPV type primers (HPV5, 8, 12, 14, 48 and 50) in the group was verified. e Specificity detection of rare type group. . The specificity of the six HPV type primers (HPV9, 63, 94, 95, 115 and 160) in the group was verified. f Microfluidic chip diagram

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