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Fig. 3 | Virology Journal

Fig. 3

From: Comparative effects of Novirhabdovirus genes on modulating constitutive transcription and innate antiviral responses, in different teleost host cell types

Fig. 3

Comparative modulation of host innate antiviral response by single IHNV genes. Epithelial (a EPC; b RTgill-W1) and fibroblastic (c BF-2; d RTG-2) cell lines were co-transfected with rainbow trout Type I IFN/luc, with MAVS as a basal IFN expression stimulator, plus two doses of each IHNV gene plasmid. Closed circular empty pcDNA3.1 plasmid vector was used for transfection balancing and baseline control. Luciferase activity was analyzed at 72 hpt and RLU normalized to total protein concentration in each sample. Data are representative of three independent experiments. Values are group means ±SEM. *p < 0.05; **p < 0.01; ***p < 0.001 indicate significant differences from pcDNA control values as determined by one-way ANOVA and Fisher’s LSD test

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