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Fig. 4 | Virology Journal

Fig. 4

From: Rapid and visual detection of milk vetch dwarf virus using recombinase polymerase amplification combined with lateral flow strips

Fig. 4

Comparison of PCR-LFS and RPA-LFS assays for detecting MDV in crude leaf extracts of field samples. Nine field samples without viral symptoms, eight field samples with mild viral symptoms, six samples with dwarfing and leaf wrinkling symptoms and healthy cowpea plants without disease symptoms were tested by PCR-LFS and RPA-LFS. PCR-LFS and RPA-LFS assays gave identical results. Of the twenty-three field samples tested, seventeen were positive and six were negative. a Detection of MDV in field samples by PCR-LFS; b Detection of MDV in field samples by RPA-LFS. N: samples without viral symptoms; M: samples with mild viral symptoms; S: samples with dwarfing and leaf wrinkling symptoms; CK: healthy cowpea plants without disease symptoms, negative control

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Virology Journal

ISSN: 1743-422X