Fig. 3
Myoma invasion assay. The Cytokeratin stained figures of HSC-3, SiHa and CaSki (a) are presented at 50 x magnification. Arrows have been added in SiHa to represent the small invaded islet (“buds”), also the area shown has been enlarged below to clearly depict the invasion. The invasion depth (b), was 1.25 μm in HSC-3, 1.28 μm in SiHa and 0.91 μm in CaSki. The difference between SiHa and CaSki was statistically significant (p < 0.01) in the invasion depth without irradiation. The invasion area (c) had also some differences. The invasion area in HSC-3 was 86.2 mm2, 94.3 mm2 in SiHa and 74.9 mm2 in CaSki. Significant differences were observed between HSC-3 and CaSki (p < 0.05) and SiHa and CaSki (p < 0.05). The statistically significant differences have been marked in the figures by adding the asterisks (*p ≤ 0.001)