TY - JOUR AU - Suzuki, Keita AU - Huits, Ralph AU - Phadungsombat, Juthamas AU - Tuekprakhon, Aekkachai AU - Nakayama, Emi E. AU - van den Berg, Riemsdijk AU - Barbé, Barbara AU - Cnops, Lieselotte AU - Rahim, Rummana AU - Hasan, Abu AU - Iwamoto, Hisahiko AU - Leaungwutiwong, Pornsawan AU - van Esbroeck, Marjan AU - Rahman, Mizanur AU - Shioda, Tatsuo PY - 2020 DA - 2020/07/02 TI - Promising application of monoclonal antibody against chikungunya virus E1-antigen across genotypes in immunochromatographic rapid diagnostic tests JO - Virology Journal SP - 90 VL - 17 IS - 1 AB - Three different genotypes of chikungunya virus (CHIKV) have been classified: East/Central/South African (ECSA), West African (WA), and Asian. Previously, a rapid immunochromatographic (IC) test detecting CHIKV E1-antigen showed high sensitivity for certain ECSA-genotype viruses, but this test showed poor performance against the Asian-genotype virus that is spreading in the American continents. We found that the reactivity of one monoclonal antibody (MAb) used in the IC rapid diagnostic test (RDT) is affected by a single amino acid substitution in E1. Therefore, we developed new MAbs that exhibited specific recognition of all three genotypes of CHIKV. SN - 1743-422X UR - https://doi.org/10.1186/s12985-020-01364-4 DO - 10.1186/s12985-020-01364-4 ID - Suzuki2020 ER -