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Fig. 3 | Virology Journal

Fig. 3

From: Small molecules block the interaction between porcine reproductive and respiratory syndrome virus and CD163 receptor and the infection of pig cells

Fig. 3

Evaluating the Inhibitory Effect on PRRSV Infection by Compounds Structurally Similar to B7. a Molecular structures of B7 analogues (B7-A1 to A6) compared with B7. b BiFC assay between SRCR5-VN and GP2a-VC proteins. Representative fluorescent images showing different effects between compound B7 and its analogues on the SRCR5-GP2a PPI, with DMSO as the Ctrl. Bar = 250 μm. c Relative fluorescence intensity in (b) quantified by Fiji. Mean ± SD, n = 3. **: P < 0.01, ***: P < 0.001. ns: non-significant. d qRT-PCR for PRRSV in total RNAs extracted from PRRSV strain VR-2332 infected PAMs treated with 15 μM of B7 and its analogues. Values are normalized with GAPDH of PAMs. Bars = mean ± SD, n = 3. *: P < 0.05, **: P < 0.01. Titration assay results for PRRSV in the culture media of PAMs treated as described in (d). Bars = mean ± SD, n = 3. *: P < 0.05, **: P < 0.01. ns: non-significant

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