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Fig. 1 | Virology Journal

Fig. 1

From: Small molecules block the interaction between porcine reproductive and respiratory syndrome virus and CD163 receptor and the infection of pig cells

Fig. 1

Establishing BiFC Assays and Screening Compounds that Inhibit the PPI Between PRRSV and CD163. a Scheme diagram for the BiFC assay fusion protein constructs between Pig CD163 SRCRs or PRRSV minor envelope glycoproteins and the fragments of Venus protein VN155(I152L) or VC155), respectively. b Left: SRCR5-VN or SRCR2-VN plasmid was co-transfected with GP2a-VC to HEK293T cells, with fluorescent images taken at 24 h after transfection. Bar = 250 μm. Right: Cell fluorescence quantified by Fiji. Mean ± SD. n = 3. **: p < 0.01. c Left: SRCR5-VN or SRCR2-VN plasmid was co-transfected with GP4-VC to HEK293T cells, with fluorescent images taken at 24 h after transfection. Bar = 250 μm. Right: Relative fluorescence intensity quantified. Mean ± SD. n = 3. **: p < 0.01. d Chemical structure of compound B7. e Left: BiFC assay between SRCR5-VN and GP2a-VC proteins. Images showing positive inhibitory effect of compound B7 but not B8, with DMSO as the Ctrl. Bar = 250 μm; Right: Relative fluorescence intensity quantified. Mean ± SD, n = 3. f Left: BiFC assay between SRCR5-VN and GP4-VC proteins showing similar inhibitory effect by B7 compound but not by B8. Bar = 120 μm. Right: Relative fluorescence intensity quantified. Mean ± SD, n = 3

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