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Fig. 3 | Virology Journal

Fig. 3

From: A ligation and restriction enzyme independent cloning technique: an alternative to conventional methods for cloning hard-to-clone gene segments in the influenza reverse genetics system

Fig. 3

The PB2 positive colonies of Bangladesh/2014 H9N2 virus were screened by plasmid PCR using the PB2 primers [31]. Out of 47 colonies screened (lane 1–47) for Bangladesh/2014 PB2, 7 were positive in plasmid PCR (indicated by arrows). The positive clones were further confirmed by nucleotide sequencing using T7F and BGHR primers. (+ = positive control; M = DNA marker)

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Virology Journal

ISSN: 1743-422X