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Fig. 5 | Virology Journal

Fig. 5

From: Influenza A virus NS1 optimises virus infectivity by enhancing genome packaging in a dsRNA-binding dependent manner

Fig. 5

Involvement of RBD of NS1 in viral replication. a 293 T RIG-I KO cells were infected with PR8 delNS1 at MOI 0.01 and transfected with empty vector (E.C.), NS1 WT or NS1 R38A as described in Fig. 2a. Cell lysate and purified virion were analysed by immunoblotting. b Viruses released in the culture supernatant were quantified by plaque assay. The Student’s t test was used for statistical analysis (n.s. not significant, **P < 0.01). The data shown are the mean ± standard deviation from at least three independent experiments (n = 3). c 293 T RIG-I KO cells were used to produce VLP as described in Fig. 3a in the absence of NS1 or NS1 WT or NS1 R38A. Proteins in the donor cells or purified VLP were examined by immunoblotting. d vRNA extracted from VLP was quantified by RT-qPCR. Levels of Firefly luciferase vRNA obtained from E.C. transfected cells were set to 1. The Student’s t test was used for statistical analysis (n.s. not significant, *P < 0.05). The data shown are the mean ± standard deviation from at least three independent experiments (n = 3). e VLP was transferred to the recipient cells (293 T RIG-I KO, Fig. 4a) and luciferase activity was measured. The Student’s t test was used for statistical analysis (n.s. not significant, **P < 0.01). The data shown are the mean ± standard deviation from at least three independent experiments (n = 3)

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