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Fig. 4 | Virology Journal

Fig. 4

From: Influenza A virus NS1 optimises virus infectivity by enhancing genome packaging in a dsRNA-binding dependent manner

Fig. 4

Effects of NS1 on gene transduction and infection by VLP in the recipient cells. a Schematic representation of VLP production and minigenome gene transduction and infection in the recipient cells. b VLP produced in the culture supernatant was transferred to the recipient cells derived either from WT 293 T or 293 T RIG-I KO cells. Luciferase activity was measured after 8 h. The Student’s t test was used for statistical analysis (*P < 0.05, **P < 0.01). The data shown are the mean ± standard deviation from at least three independent experiments (n = 3). c VLP-treated recipient cells (293 T RIG-I KO) were stained with anti-luciferase antibody (red) and DAPI (blue), and examined by confocal microscopy (left). Scale bar, 10 μm. Cells were quantified for luciferase expression (right). The Student’s t test was used for statistical analysis (*P < 0.05). The data shown are the mean ± standard deviation from at least three independent experiments (n = 3)

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