Fig. 1

IAV replication in WT and RIG-I KO cells. a 293 T RIG-I KO cells were mock treated or infected with indicated viruses at MOI = 0.1. Twenty-four hours after infection, extracted total RNA was reverse-transcribed using random primers followed by qPCR with primers targeting IFNB1. WT 293 T (b) and RIG-I KO cells (c) were infected with PR8-WT or PR8-delNS1 at MOI 0.01. The virus yield was measured at 48 h post-infection by plaque assay using MDCK cells. The Student’s t test was used for statistical analysis (**P < 0.01, *P < 0.05). The data shown are the mean ± standard deviation from at least two independent experiments (n = 2 in (a), n = 3 in (b) & (c))