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Table 1 Comparison of the three ELISA kits used for HDV detection

From: Performance of commercially available anti-HDV enzyme-linked immunosorbent assays in Taiwan

 

GB HDV Ab

DiaPro HDV Ab

DiaSorin ETI-AB-DELTAK-2

Manufacturer

General Biological Corporation

DiaPro

DiaSorin

Certification

TFDA MHW Medical Device Manufacturing No. 005807

CE 0318

CE 0459

Sample (volume)

serum and EDTA-plasma(100 μl)

serum and EDTA-plasma

(100 μl)

serum only (50 μl)

Principle

direct sandwich assay

two-step competitive assay

simultaneous competitive assay

Adsorbed Antigen

recombinant HDV antigen

recombinant HDV antigen

recombinant HDV antigen

HRP Conjugate

HDV small antigen

polyclonal Ab against HDV

human anti-HD Fab

Incubation Time

105 min

(60, 30, 15 min)

140 min

(60, 60, 20 min)

210 min

(180, 30 min)

Incubation Temperature

37 °C

37 °C or room temperature

37 °C orroom temperature

Reader Wavelength

450 nm (reading)

650 nm (blanking, if possible)

450 nm (reading)

620–630 nm (blanking, if possible)

450 nm (reading)

630 nm (blanking)

Control Well(s)

6 (Blank*1, NC*3, PC*2)

7 (Blank*1, NC*3, CAL*2, PC*1)

6 (Blank*1, NC*3, PC*2)