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Fig. 4 | Virology Journal

Fig. 4

From: The molecular evolutionary characteristics of new isolated H9N2 AIV from East China and the function of vimentin on virus replication in MDCK cells

Fig. 4

Over-expression of vimentin enhanced H9N2 virus replication. a Vimentin gene was cloned by PCR; 1 ~ 6: PCR sample 1 ~ 6. b The enzyme digestion of the positive recombinant plasmid pcDNA3.0-Vimentin. c Over-expressed vimentin protein analyzed by western blot in MDCK cells transinfected with plasmid pcDNA3.0-Vimentin. d Grayscale analysis of over-expressed vimentin protein (b). e H9N2 virus RNA replication detected by RT-qPCR. *, p < 0.05, and **, p < 0.01

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Virology Journal

ISSN: 1743-422X