Complete genome analysis of hepatitis B virus in Qinghai-Tibet plateau: the geographical distribution, genetic diversity, and co-existence of HBsAg and anti-HBs antibodies

Liu, He; Shen, Liping; Zhang, Shuang; Wang, Feng; Zhang, Guomin; Yin, Zundong; Qiu, Feng; Liang, Xiaofeng; Wang, Fuzhen; Bi, Shengli

doi:10.1186/s12985-020-01350-w

Virology Journal

Table 3 Main nucleotide and amino acid mutations of HBV CD1 and CD2 isolates in Qinghai-Tibet Plateau

From: Complete genome analysis of hepatitis B virus in Qinghai-Tibet plateau: the geographical distribution, genetic diversity, and co-existence of HBsAg and anti-HBs antibodies

Position	Nucleotide mutation	Amino acid mutation	CD1(130 isolates)		CD2(49 isolates)		Total (179 isolates)		P value
BCP	T1753C		6	4.62%	9	18.37%	15	8.38%	0.003
nt1742–1849	A1762T		35	26.92%	18	36.73%	53	29.61%	0.2
	G1764A		38	29.23%	18	36.73%	56	31.28%	0.334
	A1762T + G1764A		32	24.62%	18	36.73%	50	27.93%	0.107
PreC/C region	G1896A	W28*	17	13.08%	3	6.12%	20	11.17%	0.188
nt1814–2452	G1899A	G29D	5	3.85%	1	2.04%	6	3.35%	0.55
	A1984G		4	3.08%	38	77.55%	42	23.46%	< 0.001
	C1990T		0	0	21	42.86%	21	11.73%	< 0.001
	C2002T		2	1.54%	0	0	2	1.12%	0.383
	A2075G	I88V	6	4.62%	0	0	6	3.35%	0.126
	A2159G	S116G	3	2.31%	3	6.12%	6	3.35%	0.206
	A2189C	I126L	13	10%	10	20.41%	23	12.85%	0.064
	C2198A	L129I	9	6.92%	5	10.20%	14	7.82%	0.466
	C2288A	P159T	27	20.77%	15	30.61%	42	23.46%	0.166
PreS/S gene	T53C	F130L	5	3.85%	3	6.12%	8	4.47%	0.511
nt2881–835	T150AC	L162PQ	121	93.08%	10	20.41%	131	73.18%	< 0.001
	C129T	S155L	19	14.62%	0	0	19	10.61%	0.005
	G774A	S207N	43	33.08%	0	0	43	24.02%	< 0.001
	C3026T	A49V	8	6.15%	5	10.20%	13	7.26%	0.352
	T3098C	I73T	3	2.31%	0	0	3	1.68%	0.284
	C3121A	P81T	8	6.15%	0	0	8	4.47%	0.076
	C3189A	D103E	4	3.08%	0	0	4	2.23%	0.214
P gene	A2375C	E23D	6	4.62%	41	83.67%	47	26.26%	< 0.001
nt2307–1623	A2502T	T66S	3	2.31%	17	34.69%	20	11.17%	< 0.001
	C2523G	E73Q	2	1.54%	32	65.31%	34	18.99%	< 0.001
	A37G	S316G	113	86.92%	4	8.16%	117	65.36%	< 0.001
	G49A	V320I	10	7.69%	45	91.84%	55	30.73%	< 0.001
	G886A	V599I	104	80%	0	0	104	58.1%	< 0.001
	G899A	W603Y	0	0	49	100%	49	27.37%	< 0.001
	C930A	Q617DH	4	3.08%	49 ^a	100%			–
	T1249G	V720L	2	1.54%	46 ^b	93.88%%			–
	T1351G	S754A	0	0	47	95.92%	47	26.26%	< 0.001
	G1484A	C798Y	0	0	48	97.96%	48	26.82%	< 0.001
	C2712AT	H136YN	97	74.62%	5	10.20%	102	56.98%	< 0.001
	C3051T	P249S	120	92.31%	8	16.33%	128	71.51%	< 0.001
	A3057G	T251A	56	43.08%	3	6.12%	59	32.96%	< 0.001
	A3108G	S268G	43	33.08%	2	4.08%	45	25.14%	< 0.001
	A942T	L613QH	129	99.23%	48 ^c	97.96%%			–
	T3210A	S272TN	130	100%	49	100%	179	100%	–
X gene	G1386AC	V5ML	31	23.85%	0	0	31	17.32%	< 0.001
nt1374–1838	G1515A, A1516C	D48AN	20	15.38%	0	0	20	11.17%	0.04
	G1613A		14	10.77%	2	4.08%	16	8.94%	0.162
	C1653T	H94Y	7	5.38%	3	6.12%	10	5.59%	0.848
	T1633A	Q87L	2	99.23%	46	93.88%	48	26.82%	< 0.001
	T1485C	S38P	129	0.77%	49	100%	178	99.44%	–

a Amino acid mutation E617DH for CD2
b Amino acid mutation L720VS for CD2
c Amino acid mutation H613K for CD2
* means stop condon
— means P value could not be calculated

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ISSN: 1743-422X

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