Table 1 List of primers used to amplify the different regions of the HBV genome and their respective thermal profile

BCP/Precore region

First

BcpF1(1254–1273)

TCCTCTGCCGATCCATACTG

80 °C(3 min); 95 °C (40 s), 63 °C (1 min), 71 °C (2.5 min)/40 cycle; 72 °C(7 min)

BcpR1(2416–2439)

TTCCCGAGATTGAGATCTTCTGCG

Second

BcpF2(1606–1625)

GCATGGAGACCACCGTGAAC

95 °C(5 min); 95 °C (40 s), 50 °C (30 s), 72 °C (1 min)/35 cycle; 72 °C(7 min)

BcpR2(1955–1974)

GGAAAGAAGTCAGAAGGCAA

Complete genome (without BCP)

First

HBV1799FLong(1799–1826)

CTGCGCACCAGCACCATGCAACTTTTTC

80 °C (3 min); 95 °C (40 s), 58 °C (1.5 min), 68 °C (4 min)/45 cycle; 72 °C(7 min)

HBV1801RLong(1774–1801)

CAGACCAATTTATGCCTACAGCCTCCTA

Second

1

HBV1847FS (1847–1867)

TGTTCATGTCCCACTGTTCAA

95 °C(5 min); 95 °C (40 s), 63 °C (30 s), 72 °C (1 min)/35 cycle; 72 °C(7 min)

HBV2394RS (2394–2408)

GGCGAGGGAGTTCTT

2

HBV2298FS (2298–2315)

GACCACCAAATGCCCCTAT

HBV2933RS (2933–2954)

TCGGGAAAGAATCCCAGAGGAT

3

HBV2821FS (2821–2840)

GGTCACCATATTCTTGGGAAC

HBV272RS (272–291)

TGAGAGAAGTCCACCACGAGT

4

HBV179FS (179–197)

CTAGGACCCCTGCTCGTGTT

HBV704RS (704–725)

CGAACCACTGAACAAATGGCACT

5

HBV0599FS(599–619)

GTATTCCCATCCCATCATCCTG

HBV1286RS (1286–1305)

GCTAGGAGTTCCGCAGTATGG

6

HBV1175FS (1175–1190)

GCCAAGTGTTTGCTGA

HBV1788RS(1788–1802)

GCCTACAGCCTCCTA

SP6

CGATTTAGGTGACACTATAGAAGAGAGGCT

T7

CAGTAATACGACTCACTATAGGGAGAAGGCT