Fig. 6

SOCS1 mediates the inhibition of IFNs response. (a and b) 293 T cells were transfected with siRNA 1 targeting SOCS1or NC siRNA. 24 h later, cells were left uninfected or infected with IAV at an MOI of 1. Twenty-four hours post infection, cells were left untreated (−) or treated with human IFN-α (1000 U/ml) (a) or IFN-γ (1000 U/ml) (b) for 1 h, and the protein level of JAK1, pSTAT1, SOCS1, viral NP and β-actin were analyzed using Western blotting. (c and d) 293 T cells were transfected with siRNA 1 targeting SOCS1or NC siRNA. Six hours later, 293 T cells were left uninfected or infected with IAV at an MOI of 1. At 24 hpi, cells were left untreated (−) or treated with human IFN-α (1000 U/ml) for 24 h or IFN-γ (1000 U/ml) for 6 h. The relative mRNA levels of ISG56 (c) or LMP-2 (d) were analyzed using real-time qPCR. The error bars represent the means plus standard deviations for three independent experiments. *, p ≤ 0.05