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Fig. 2 | Virology Journal

Fig. 2

From: Generation and immunogenicity assessment of ELPylated virus-like particles of porcine circovirus type 2

Fig. 2

Purification of ELP-Cap and Cap-His fusion proteins. a The total cell lysate of pELP-Cap recombinant E. coli (1) and ELP-Cap fusion protein (2) purified by ITC. b Purification of ELP-Cap protein by ITC in the presence of different concentrations of Triton X-100. c Purification of ELP-Cap protein by ITC in the presence of different concentrations of urea. d The total cell lysate of pELP-Cap recombinant E. coli (1) and ELP-Cap protein (2) purified by ITC in the presence of 1% Triton X-100 and 0.5 M urea. e The total cell lysate of pET-Cap recombinant E. coli (1) and Cap-His fusion protein (2) purified by nickel affinity chromatography. All samples were analyzed by 12% SDS-PAGE

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Virology Journal

ISSN: 1743-422X