Fig. 1

Overview of the experimental procedure. a Overview of TG collection and RNA-sequencing data used in this study. Mouse and Tree Shrew infected models were established through the ocular infection route, following which TGs were collected at different infection times for RNA sequencing. In addition to 16 published human TG data sets, we generated RNA-sequencing data sets from 4 human TGs. These RNA-seq data were used for viral transcriptome analysis in this study. Sample types were labeled in each host model: uninfected samples, acute infected samples and latent infected samples. b Experimental timeline for infection of animals and the subsequent collection of samples. c Line chart representing the total number of viral genes (read count > 10) at the indicated time points. Data are represented as mean ± standard deviation (SD), and means were labeled around points. d Distribution of absolute log₂ fold changes for 74 viral genes between adjacent time points in two animal models. Lines represent medians, boxes represent 25–75% intervals, and whiskers represent 5–95% intervals. Outliers are not shown. Differences in fold changes were tested by ANOVA. e The lines report number of DEGs (log₂ fold change > 1 & adjusted P value < 0.01) for each pair of consecutive time points in two animal modes