Skip to main content
Fig. 4 | Virology Journal

Fig. 4

From: Three kinds of treatment with Homoharringtonine, Hydroxychloroquine or shRNA and their combination against coronavirus PEDV in vitro

Fig. 4

Antiviral activity of combination treatment. a-b, Vero cells seeded at 2.5 × 106 cells per well in six-well plates were infected with PEDV at 0.1 MOI in the presence of the HHT at different concentrations for 48 h, electrophoretically separated proteins were analyzed by immunoblotting with antibodies to viral protein PEDV-N and actin (a). Viral yields in the medium were determined by TCID50 (b). These experiments were performed two times with three replicates in each experiment. Values represent means and SD. * p < 0.05; ** p < 0.01; *** p < 0.001 compared to PBS-treated group. c-d, Vero cells seeded at 2.5 × 106 cells per well in six-well, cells were then infected with PEDV at 0.1 MOI in the presence of peptide tHR2 and HHT (c) or HCQ (d). At 48 h post-infection, virus titer in TCID50 was determined. e-g, Cytotoxicity of HHT and combination treatment was detected by MTT assay. These experiments were performed two times with three replicates in each experiment. Values represent means and SD for triplicates. *p < 0.05, **p < 0.01 for one-way ANOVA

Back to article page