Fig. 9

Overexpression of PD-L1 suppresses T cell function. RPMECs were transfected with control plasmid (control) or a PD-L1 CRISPR activation plasmid (plasmid) for 48 h, and then cells were inoculated with viral particles at an MOI of 5 for 24 h. Then, the activated T cells were added to the RPMECs for 8 h in vitro. Migrated T cells were collected and cultured for flow cytometry and ELISA. The overexpression of PD-L1 was detected by western blotting. a PD-L1 protein levels in RPMECs after plasmid transfection. b The levels of granzyme B in the culture supernatant of T cells. c The percentage of perforin-positive T cells. * indicates the plasmid group compared to the control group (**P < 0.01, t-test)