Fig. 4
 Determination of transmigrated T cells. RPMECs were cultured on transwell filters, and confluence was assessed by measuring the fluorescence intensity in the lower chamber. T cells (1 × 106 cells per well) were added to the top chamber and allowed to transmigrate for 8 h. Then, the transmigrated T cells were counted by detecting fluorescence intensity. a The confluence of RPMECs on days 0, 1, 2 and 3. b The numbers of transmigrated T cells. T cells from H9N2+rat indicated that T cells were isolated from H9N2 virus-infected rats. T cells from H9N2-rat indicated that T cells were isolated from rats with the inactivated H9N2 viral particles. * Statistical analysis of RPMECs confluence at different time points (***P < 0.001, t-test)