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Fig. 5 | Virology Journal

Fig. 5

From: Mitomycin, 5-fluorouracil, leflunomide, and mycophenolic acid directly promote hepatitis B virus replication and expression in vitro

Fig. 5

Lamivudine susceptibility assay. Lamivudine inhibits the activation of HBV replication in HepG2.2.15 cells treated with epirubicin, mitomycin, 5-fluorouracil, dexamethasone, leflunomide, and mycophenolic acid. HepG2.2.15 cells were treated with increasing concentrations of lamivudine for 24 h and then treated with 1 μM epirubicin (a), 1 μM mitomycin (b), or 100 μM 5-fluorouracil (c), for 16 h, or 10 μM dexamethasone (d), 50 μM leflunomide (e), or 30 μM mycophenolic acid (f), for 5 days. After incubation with increasing concentrations of lamivudine for 24 h, the cells were cultured in fresh media without drugs. Subsequently, intracellular capsid DNA was detected by molecular hybridization with the α-32P-labeled HBV DNA probe after NAGE (top). The number of extracellular HBV DNA copies was measured by qPCR (bottom), and the mean values from three isodose treatments, including SD, were statistically significant (p < 0.05)

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