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Table 1 Primers for the construction of full-length cDNA clone of BEV

From: Experimental immunization of mice with a recombinant bovine enterovirus vaccine expressing BVDV E0 protein elicits a long-lasting serologic response

Primer name

Primer sequence (5′ to 3′)

Product size (bp)

BEV-AF

GTAAAACGACGGCCAGTGAATTGGTCGACTAATACGACTCACTATAGGGTTAAAACAGCCTGGGGGTTG

3383

BEV-AR

GGGCCCAATGTGGTCAGGGAGGATCGATCGGTGCAGATCTTATCGGTG

BEV-BF

GCACCGATCGATCCTCCCTGACCACATTGGGCCCATTTGGAC

2969

BEV-BR

GCCTTCAGTACCATAAATGGCATCCTCCATGGATATCGGCCTAGG

BEV-CF

CTAAATATTGATCCTAGGCCGATATCCATGGAGGATGCC

1249

BEV-CR

CTCGAAATTAACCCTCACTAAAGGGAACGAATTCTTTTTTTTTTTTTTTTTTACACCCCATCCGGTG

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Virology Journal

ISSN: 1743-422X