Fig. 1

Construction and strategy of the GpBSK-BEV and GpBSK-BEV-E0 plasmids. (a) Construction of the GpBSK-BEV plasmid; (b) The insertion of NotI and XbaI double restriction sites and a recognition site for 3Cpro into the 2C/3A junction in the GpBSK-BEV plasmid; (c) Construction of the GpBSK-BEV-E0 plasmid, the 3Cpro cleavage site (ALPQG) and NotI and XbaI enzyme double restriction sites are underlined