Fig. 4
The effect of PIAS4 expression to C protein expression and viral titer. a Knockdown of PIAS4 protein levels by siRNA treatment. MDBK cells were transfected with no siRNA (Mock), scramble siRNA (siScr), siRNA targeting PIAS4 and harvested at 48 hpt. The PIAS4 and C protein were detected by immunoblotting. b BVDV titers in PIAS4 knockdown cells. c Increased C protein expression in PIAS4-overexpressing cells. MDBK cells were transfected with pCDNA3.1-myc-PIAS4 (PIAS4) and empty vector (EV) for 12 h, followed by infection with BVDV for 48 h. The expression levels of C-protein and PIAS4 were analyzed by WB. d Increased BVDV growth in PIAS4-overexpressing cells. The detection of virus titers was described above. The virus titers in the supernatants of all treatment groups were determined by virus titration assays and expressed as TCID50/0.1 ml. Error bars represent the standard error of the mean from three independent experiments. *P < 0.05, **P < 0.01 were considered statistically significant