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Fig. 4 | Virology Journal

Fig. 4

From: Disulfide isomerase ERp57 improves the stability and immunogenicity of H3N2 influenza virus hemagglutinin

Fig. 4

The percentage of the disulfide-bonded HA trimers significantly descended when the ERp57 proteins were down-expressed. a Real-time qPCR analysis of ERp57 gene expression. iERp57 denotes the expression of ERp57 genes in the ERp57 gene-knockdowned cells. ** represents P < 0.01. b Western-blot analysis of ERp57 expression under reducing condition; iERp57 denotes the expression of ERp57 proteins in the ERp57 gene-knockdowned cells. c Western-blot analysis of HA expression under reducing condition; Mock denotes the conventional 293 T cells transfected by blank plasmid; HA denotes the expression of HA proteins in the conventional 293 T cells; NC denotes the expression of HA proteins in the 293 T cells transfected by negative control siRNA which don’t interference any genes expression in the 293 T cells; iERp57 denotes the expression of HA proteins in the ERp57 gene-knockdowned cells. d Western-blot analysis of the disulfide-bonded HA trimers under non-reducing condition; iERp57 denotes the expression of HA proteins in the ERp57 gene-knockdowned cells. e A statistical graph of the disulfide-bonded HA trimers analyzed by ImageJ. *** represents P < 0.001

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