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Fig. 2 | Virology Journal

Fig. 2

From: Disulfide isomerase ERp57 improves the stability and immunogenicity of H3N2 influenza virus hemagglutinin

Fig. 2

Expression and characterization of HA proteins in 293 T cells. HA denotes the single expression of H3 HA proteins; PDI/HA denotes co-expression of PDI and H3 HA proteins; ERp57/HA denotes co-expression of ERp57 and H3 HA proteins. a Western-blot analysis of HA protein expression under reducing condition. ImageJ was used to test the quantity of HA proteins. b Indirect immunofluorescence assay of HA protein expression. Cells nuclei were stained with DAPI (blue), and HA proteins were reacted with primary antibody and Cy3-conjugated secondary antibody (red). Scale bars indicate 25 μm. c Flow cytometry analysis of HA protein expression

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Virology Journal

ISSN: 1743-422X